Place a small spot of paint on the center of your bicycle's front tire.
Move the bicycle forward so that the tire makes two paint marks on the pavement. As the late bicycling expert Sheldon Brown notes, for accuracy's sake, you must keep the wheel perfectly straight when moving forward. Use an assistant to steady and guide the bike, while you, sitting on the bike, hold the handlebar straight.
Measure the distance between the two paint marks the tire makes on the road with a tape measure. For greater accuracy, Brown advises moving the bike three to four revolutions and using the average distance between all revolutions.
Calculate your result by 2.54 to get centimeters and 25.4 to get millimeters if your tape measure uses inches. Some bike computers require you to input tire size in centimeters, while others use millimeters. If your computer asks for radius, divide your result by 6.2832 to obtain that value.